Genetic diversity in Cucurbita pepo landraces from northern. It appears that hydroxylation is a control point in carotenoid biosynthesis. moschata were beta-carotene and alpha-carotene, whlereas lutein and beta-carotene dominate in C. Combining data from the current study with those in the literature, profiles for the Cucurbita species could be observed. Cucurbita maxima 'ExposiÃ§Ã£o' had a different profile, with the predominance of violaxanthin, followed by beta-carotene and lutein. The hybrid 'Tetsukabuto' resembled the Cucurbita pepo 'Mogango', lutein and beta-carotene being the principal carotenoids.

moschata 'Goianinha' had similar profiles, with beta-carotene and alpha-carotene as the major carotenoids. Cucurbita moschata 'Menina Brasileira' and C. In the present work, the carotenoid composition of squashes and pumpkins currently marketed in Campinas, Brazil, were determined by HPLC-DAD, complemented by HPLC-MS for identification. The carotenoid composition has been determined, but reported data have been highly variable, both qualitatively and quantitatively. Squashes and pumpkins are important dietary sources of carotenoids worldwide. Qualitative and quantitative differences in carotenoid composition among Cucurbita moschata, Cucurbita maxima, and Cucurbita pepo.Īzevedo-Meleiro, Cristiane H Rodriguez-Amaya, Delia B

(MRS), Nutrient agar (NA) and Potato dextrose. good storage characteristics and affordable cost. properties of pumpkin ( Cucurbita pepo) and sorghum (Sorghum bicolor) flour blends fermented with pure strains of Lactobacillus. These observations were discussed with respect to satellite DNA evolution and compared to the data received from monocotyledonous species. pepo genome a high amount of sequences resembling the satellite of C. The two satellite DNAs showed no cross-hybridization to each other and sequence homology is only limited.

The sequence heterogeneity between these variants is about 15%. This satellite is also rich in AT-base pairs (64%), existing in at least three different variants as revealed by restriction enzyme analysis and DNA sequencing. In contrast, the satellite DNA of Cucurbita maxima has a repeat length of 168-169 base pairs. This satellite is highly conserved in restriction enzyme pattern and DNA sequence sequence heterogeneity is about 10%. The percentage of AT-base pairs is about 61%. DNA sequencing revealed that the repeat length of satellite DNA in Cucurbita pepo is 349-352 base pairs. The AT-rich highly repeated satellite DNA of Cucurbita pepo (zucchini) and Cucurbita maxima (pumpkin) were cloned and their DNA structure was investigated. Different AT-rich satellite DNAs in Cucurbita pepo and Cucurbita maxima.

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